河西学院学报

本研究以采自祁连山的一株野生马鞍菌为材料，通过组织分离并纯化得到菌株HL-01，采用形态学观察结合ITS序列分子生物学方法进行物种鉴定，同时检测不同温度、pH、碳源、氮源、碳氮比和金属离子6个不同因素对HL-01菌丝的影响，选取4个影响显著的因素开展正交试验。结果表明：HL-1为裂盖马鞍菌，其菌丝的最适生长温度为22℃，最适氮源为酵母粉，最适碳源为麦芽糖，最适碳氮比为20:1，最适pH为5，最适无机盐为硫酸锰。最佳培养条件组合为A3B3C2D3或A3B3C2D1或A3B1C2D3，即在24℃（A3）下，碳源为乳糖（B3）或可溶性淀粉（B1），氮源为酵母粉（C2），无机盐为硫酸钙（D3）或硫酸锰（D1）的培养基培养。本研究可为祁连山马鞍菌资源的的开发和种质资源的保护提供理论依据。

This study utilized a wild Helvella sp. specimen collected from the Qilian Mountains as the material. Through tissue isolation and purification,strain HL-01 was obtained. Species identification was carried out based on morphological observation combined with molecular analysis using ITS sequences. Six factors affecting the mycelial growth of HL-01 were examined,including temperature,pH,carbon source,nitrogen source,carbon-to-nitrogen ratio,and metal ions. Four factors with significant effects were selected for orthogonal experiments. The results showed that HL-01 was identified as Helvella leucopus. The optimal growth conditions for its mycelia were:a temperature of 22℃,yeast extract as the nitrogen source,maltose as the carbon source,a carbon-to-nitrogen ratio of 20:1,pH 5,and manganese sulfate as the optimal inorganic salt. The best combination of culture conditions was A3B3C2D3,A3B3C2D1,or A3B1C2D3,which corresponded to cultivation at 24℃(A3),using lactose(B3)or soluble starch(B1)as the carbon source,yeast extract(C2)as the nitrogen source,and calcium sulfate(D3)or manganese sulfate(D1)as the inorganic salt. This study provides a theoretical basis for the development and germplasm conservation of Helvella resources in the Qilian Mountains.